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function blocking antibody against scf  (R&D Systems)


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    R&D Systems function blocking antibody against scf
    A. Western blot of AsPC1 pancreatic cancer cells expressing control vector (V) or Snail (Sn). B. The mRNA samples from AsPC1-V and AsPC1-Sn cells were analyzed for stem cell factor <t>(SCF)</t> and GAPDH using real-time PCR, and the mRNA levels normalized to the levels present in vector-expressing control cells (*** p<0.001). AsPC1-VCM and AsPC1-SnCM were generated as detailed in Materials and Methods. SCF protein levels in the conditioned media were determined using the human Quantikine ELISA kit DCK00 purchased from R&D Systems (**, p<0.01). C. AsPC1-V and AsPC1-Sn cells were treated with either DMSO or TGF-β type I receptor inhibitor SB431542 for 72 hours, and the effect on stem cell factor (SCF) mRNA level was determined by qRT-PCR and normalized using GAPDH mRNA levels. The mRNA levels were normalized to the levels present in DMSO-treated AsPC1-V cells (*, p<0.05). The p-values were calculated using paired t-test. E. HMC-1 mast cells (2 × 105) were added to the upper chamber of an 8 μm uncoated Boyden chamber with either AsPC1-VCM or AsPC1-SnCM supplemented with 1% serum in the lower chamber. HMC-1 cells were allowed to migrate into the lower chamber, collected and counted. The p-values were calculated using paired t-test. E. HMC-1 mast cells (2 × 105) were added to the upper chamber of an 8 μm uncoated Boyden chamber with either AsPC1-VCM or AsPC1-SnCM in the lower chamber. A neutralizing antibody against SCF (AF-255-NA) or an <t>isotype-matched</t> <t>IgG</t> antibody was added to the lower chamber. HMC-1 cells were allowed to migrate over 18 hours into the lower chamber, collected and counted.*, p <0.05. The p-values were calculated using paired t-test.
    Function Blocking Antibody Against Scf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/function blocking antibody against scf/product/R&D Systems
    Average 91 stars, based on 11 article reviews
    function blocking antibody against scf - by Bioz Stars, 2026-06
    91/100 stars

    Images

    1) Product Images from "Snail Cooperates with Kras G12D in vivo to Increase Stem Cell Factor and Enhance Mast Cell"

    Article Title: Snail Cooperates with Kras G12D in vivo to Increase Stem Cell Factor and Enhance Mast Cell

    Journal: Molecular cancer research : MCR

    doi: 10.1158/1541-7786.MCR-14-0111

    A. Western blot of AsPC1 pancreatic cancer cells expressing control vector (V) or Snail (Sn). B. The mRNA samples from AsPC1-V and AsPC1-Sn cells were analyzed for stem cell factor (SCF) and GAPDH using real-time PCR, and the mRNA levels normalized to the levels present in vector-expressing control cells (*** p<0.001). AsPC1-VCM and AsPC1-SnCM were generated as detailed in Materials and Methods. SCF protein levels in the conditioned media were determined using the human Quantikine ELISA kit DCK00 purchased from R&D Systems (**, p<0.01). C. AsPC1-V and AsPC1-Sn cells were treated with either DMSO or TGF-β type I receptor inhibitor SB431542 for 72 hours, and the effect on stem cell factor (SCF) mRNA level was determined by qRT-PCR and normalized using GAPDH mRNA levels. The mRNA levels were normalized to the levels present in DMSO-treated AsPC1-V cells (*, p<0.05). The p-values were calculated using paired t-test. E. HMC-1 mast cells (2 × 105) were added to the upper chamber of an 8 μm uncoated Boyden chamber with either AsPC1-VCM or AsPC1-SnCM supplemented with 1% serum in the lower chamber. HMC-1 cells were allowed to migrate into the lower chamber, collected and counted. The p-values were calculated using paired t-test. E. HMC-1 mast cells (2 × 105) were added to the upper chamber of an 8 μm uncoated Boyden chamber with either AsPC1-VCM or AsPC1-SnCM in the lower chamber. A neutralizing antibody against SCF (AF-255-NA) or an isotype-matched IgG antibody was added to the lower chamber. HMC-1 cells were allowed to migrate over 18 hours into the lower chamber, collected and counted.*, p <0.05. The p-values were calculated using paired t-test.
    Figure Legend Snippet: A. Western blot of AsPC1 pancreatic cancer cells expressing control vector (V) or Snail (Sn). B. The mRNA samples from AsPC1-V and AsPC1-Sn cells were analyzed for stem cell factor (SCF) and GAPDH using real-time PCR, and the mRNA levels normalized to the levels present in vector-expressing control cells (*** p<0.001). AsPC1-VCM and AsPC1-SnCM were generated as detailed in Materials and Methods. SCF protein levels in the conditioned media were determined using the human Quantikine ELISA kit DCK00 purchased from R&D Systems (**, p<0.01). C. AsPC1-V and AsPC1-Sn cells were treated with either DMSO or TGF-β type I receptor inhibitor SB431542 for 72 hours, and the effect on stem cell factor (SCF) mRNA level was determined by qRT-PCR and normalized using GAPDH mRNA levels. The mRNA levels were normalized to the levels present in DMSO-treated AsPC1-V cells (*, p<0.05). The p-values were calculated using paired t-test. E. HMC-1 mast cells (2 × 105) were added to the upper chamber of an 8 μm uncoated Boyden chamber with either AsPC1-VCM or AsPC1-SnCM supplemented with 1% serum in the lower chamber. HMC-1 cells were allowed to migrate into the lower chamber, collected and counted. The p-values were calculated using paired t-test. E. HMC-1 mast cells (2 × 105) were added to the upper chamber of an 8 μm uncoated Boyden chamber with either AsPC1-VCM or AsPC1-SnCM in the lower chamber. A neutralizing antibody against SCF (AF-255-NA) or an isotype-matched IgG antibody was added to the lower chamber. HMC-1 cells were allowed to migrate over 18 hours into the lower chamber, collected and counted.*, p <0.05. The p-values were calculated using paired t-test.

    Techniques Used: Western Blot, Expressing, Control, Plasmid Preparation, Real-time Polymerase Chain Reaction, Generated, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR



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    function blocking antibody against scf  (R&D Systems)
    91
    R&D Systems function blocking antibody against scf
    A. Western blot of AsPC1 pancreatic cancer cells expressing control vector (V) or Snail (Sn). B. The mRNA samples from AsPC1-V and AsPC1-Sn cells were analyzed for stem cell factor <t>(SCF)</t> and GAPDH using real-time PCR, and the mRNA levels normalized to the levels present in vector-expressing control cells (*** p<0.001). AsPC1-VCM and AsPC1-SnCM were generated as detailed in Materials and Methods. SCF protein levels in the conditioned media were determined using the human Quantikine ELISA kit DCK00 purchased from R&D Systems (**, p<0.01). C. AsPC1-V and AsPC1-Sn cells were treated with either DMSO or TGF-β type I receptor inhibitor SB431542 for 72 hours, and the effect on stem cell factor (SCF) mRNA level was determined by qRT-PCR and normalized using GAPDH mRNA levels. The mRNA levels were normalized to the levels present in DMSO-treated AsPC1-V cells (*, p<0.05). The p-values were calculated using paired t-test. E. HMC-1 mast cells (2 × 105) were added to the upper chamber of an 8 μm uncoated Boyden chamber with either AsPC1-VCM or AsPC1-SnCM supplemented with 1% serum in the lower chamber. HMC-1 cells were allowed to migrate into the lower chamber, collected and counted. The p-values were calculated using paired t-test. E. HMC-1 mast cells (2 × 105) were added to the upper chamber of an 8 μm uncoated Boyden chamber with either AsPC1-VCM or AsPC1-SnCM in the lower chamber. A neutralizing antibody against SCF (AF-255-NA) or an <t>isotype-matched</t> <t>IgG</t> antibody was added to the lower chamber. HMC-1 cells were allowed to migrate over 18 hours into the lower chamber, collected and counted.*, p <0.05. The p-values were calculated using paired t-test.
    Function Blocking Antibody Against Scf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/function blocking antibody against scf/product/R&D Systems
    Average 91 stars, based on 1 article reviews
    function blocking antibody against scf - by Bioz Stars, 2026-06
    91/100 stars
    		  Buy from Supplier

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    A. Western blot of AsPC1 pancreatic cancer cells expressing control vector (V) or Snail (Sn). B. The mRNA samples from AsPC1-V and AsPC1-Sn cells were analyzed for stem cell factor (SCF) and GAPDH using real-time PCR, and the mRNA levels normalized to the levels present in vector-expressing control cells (*** p<0.001). AsPC1-VCM and AsPC1-SnCM were generated as detailed in Materials and Methods. SCF protein levels in the conditioned media were determined using the human Quantikine ELISA kit DCK00 purchased from R&D Systems (**, p<0.01). C. AsPC1-V and AsPC1-Sn cells were treated with either DMSO or TGF-β type I receptor inhibitor SB431542 for 72 hours, and the effect on stem cell factor (SCF) mRNA level was determined by qRT-PCR and normalized using GAPDH mRNA levels. The mRNA levels were normalized to the levels present in DMSO-treated AsPC1-V cells (*, p<0.05). The p-values were calculated using paired t-test. E. HMC-1 mast cells (2 × 105) were added to the upper chamber of an 8 μm uncoated Boyden chamber with either AsPC1-VCM or AsPC1-SnCM supplemented with 1% serum in the lower chamber. HMC-1 cells were allowed to migrate into the lower chamber, collected and counted. The p-values were calculated using paired t-test. E. HMC-1 mast cells (2 × 105) were added to the upper chamber of an 8 μm uncoated Boyden chamber with either AsPC1-VCM or AsPC1-SnCM in the lower chamber. A neutralizing antibody against SCF (AF-255-NA) or an isotype-matched IgG antibody was added to the lower chamber. HMC-1 cells were allowed to migrate over 18 hours into the lower chamber, collected and counted.*, p <0.05. The p-values were calculated using paired t-test.

    Journal: Molecular cancer research : MCR

    Article Title: Snail Cooperates with Kras G12D in vivo to Increase Stem Cell Factor and Enhance Mast Cell

    doi: 10.1158/1541-7786.MCR-14-0111

    Figure Lengend Snippet: A. Western blot of AsPC1 pancreatic cancer cells expressing control vector (V) or Snail (Sn). B. The mRNA samples from AsPC1-V and AsPC1-Sn cells were analyzed for stem cell factor (SCF) and GAPDH using real-time PCR, and the mRNA levels normalized to the levels present in vector-expressing control cells (*** p<0.001). AsPC1-VCM and AsPC1-SnCM were generated as detailed in Materials and Methods. SCF protein levels in the conditioned media were determined using the human Quantikine ELISA kit DCK00 purchased from R&D Systems (**, p<0.01). C. AsPC1-V and AsPC1-Sn cells were treated with either DMSO or TGF-β type I receptor inhibitor SB431542 for 72 hours, and the effect on stem cell factor (SCF) mRNA level was determined by qRT-PCR and normalized using GAPDH mRNA levels. The mRNA levels were normalized to the levels present in DMSO-treated AsPC1-V cells (*, p<0.05). The p-values were calculated using paired t-test. E. HMC-1 mast cells (2 × 105) were added to the upper chamber of an 8 μm uncoated Boyden chamber with either AsPC1-VCM or AsPC1-SnCM supplemented with 1% serum in the lower chamber. HMC-1 cells were allowed to migrate into the lower chamber, collected and counted. The p-values were calculated using paired t-test. E. HMC-1 mast cells (2 × 105) were added to the upper chamber of an 8 μm uncoated Boyden chamber with either AsPC1-VCM or AsPC1-SnCM in the lower chamber. A neutralizing antibody against SCF (AF-255-NA) or an isotype-matched IgG antibody was added to the lower chamber. HMC-1 cells were allowed to migrate over 18 hours into the lower chamber, collected and counted.*, p <0.05. The p-values were calculated using paired t-test.

    Article Snippet: Function blocking antibody against SCF (AF-255-NA) and isotype-matched IgG antibody were purchased from R&D Systems.

    Techniques: Western Blot, Expressing, Control, Plasmid Preparation, Real-time Polymerase Chain Reaction, Generated, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR